bbsi hf (New England Biolabs)
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bbsi hf
Bbsi Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 626 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbsi+hf/BbsI-HF/pm42129570-619-31-32
Average 99 stars, based on 626 article reviews
Bbsi Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 626 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbsi+hf/BbsI-HF/pm42129570-619-31-32
Average 99 stars, based on 626 article reviews
bbsi hf - by Bioz Stars,
2026-09
99/100 stars
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Plasmid Preparation:Article Title: Endogenous short enhancer sequences increase expression of soybean and cowpea RUBP regeneration genes Article Snippet: Promoter constructs were generated by first amplifying genomic DNA via regular PCR and then introducing different sequences via overhang PCR using VeriFi® Hot Start Mix (PCR biosystems) or Q5® High-Fidelity DNA Polymerase (NEB)(see Supporting Data S 1 for a list of all used primers). .. The custom level 2 double fluorophore vector was generated by assembling a lacZ fragment, a fragment containing the EYFP coding sequence and an HSPt-terminator together with a fragment containing a p35S-promoter, the coding sequence of mRFP1 and a NOSt-terminator, together with a linker into pAGM4723 via Golden Gate cloning using Article Title: Developmental gene expression patterns driving species-specific cortical features. Article Snippet: Addgene #29687 pCS2-Flag-JUNB was used for JUNB overexpression experiments. .. Addgene #175573 pX458-Ef1adCas9-KRAB-MECP2-H2B-GFP (CRISPRi) was modified by replacing the Ef1a promoter with CAG using NEB HiFi DNA assembly reaction (NEB: E2621S). pCAG-CRISPRi plasmid generated in this study was then digested with Article Title: Chromatin dynamics of the Klf4 locus in mouse pluripotent cells. Article Snippet: Single stranded oligos containing the target sequence with a 5’ CACC overhang and the reverse complement of the target sequence with a 5’ AAAC overhang were annealed in 1X NEB Buffer 2.1 (New England Biolabs, Catalog no: B7202S) by heating up the sample to 95°C for 5 minutes and ramp down the temperature to 25°C by 5°C per minute in a thermocycler (final concentration 10 μM). .. To insert the target sequence in the plasmid backbones, PX459 V2.0 and B-SpCas9-HF1-2A-PuroR were digested with 1 μl Generated:Article Title: Endogenous short enhancer sequences increase expression of soybean and cowpea RUBP regeneration genes Article Snippet: Promoter constructs were generated by first amplifying genomic DNA via regular PCR and then introducing different sequences via overhang PCR using VeriFi® Hot Start Mix (PCR biosystems) or Q5® High-Fidelity DNA Polymerase (NEB)(see Supporting Data S 1 for a list of all used primers). .. The custom level 2 double fluorophore vector was generated by assembling a lacZ fragment, a fragment containing the EYFP coding sequence and an HSPt-terminator together with a fragment containing a p35S-promoter, the coding sequence of mRFP1 and a NOSt-terminator, together with a linker into pAGM4723 via Golden Gate cloning using Article Title: Developmental gene expression patterns driving species-specific cortical features. Article Snippet: Addgene #29687 pCS2-Flag-JUNB was used for JUNB overexpression experiments. .. Addgene #175573 pX458-Ef1adCas9-KRAB-MECP2-H2B-GFP (CRISPRi) was modified by replacing the Ef1a promoter with CAG using NEB HiFi DNA assembly reaction (NEB: E2621S). pCAG-CRISPRi plasmid generated in this study was then digested with Sequencing:Article Title: Endogenous short enhancer sequences increase expression of soybean and cowpea RUBP regeneration genes Article Snippet: Promoter constructs were generated by first amplifying genomic DNA via regular PCR and then introducing different sequences via overhang PCR using VeriFi® Hot Start Mix (PCR biosystems) or Q5® High-Fidelity DNA Polymerase (NEB)(see Supporting Data S 1 for a list of all used primers). .. The custom level 2 double fluorophore vector was generated by assembling a lacZ fragment, a fragment containing the EYFP coding sequence and an HSPt-terminator together with a fragment containing a p35S-promoter, the coding sequence of mRFP1 and a NOSt-terminator, together with a linker into pAGM4723 via Golden Gate cloning using Article Title: Chromatin dynamics of the Klf4 locus in mouse pluripotent cells. Article Snippet: Single stranded oligos containing the target sequence with a 5’ CACC overhang and the reverse complement of the target sequence with a 5’ AAAC overhang were annealed in 1X NEB Buffer 2.1 (New England Biolabs, Catalog no: B7202S) by heating up the sample to 95°C for 5 minutes and ramp down the temperature to 25°C by 5°C per minute in a thermocycler (final concentration 10 μM). .. To insert the target sequence in the plasmid backbones, PX459 V2.0 and B-SpCas9-HF1-2A-PuroR were digested with 1 μl Cloning:Article Title: Endogenous short enhancer sequences increase expression of soybean and cowpea RUBP regeneration genes Article Snippet: Promoter constructs were generated by first amplifying genomic DNA via regular PCR and then introducing different sequences via overhang PCR using VeriFi® Hot Start Mix (PCR biosystems) or Q5® High-Fidelity DNA Polymerase (NEB)(see Supporting Data S 1 for a list of all used primers). .. The custom level 2 double fluorophore vector was generated by assembling a lacZ fragment, a fragment containing the EYFP coding sequence and an HSPt-terminator together with a fragment containing a p35S-promoter, the coding sequence of mRFP1 and a NOSt-terminator, together with a linker into pAGM4723 via Golden Gate cloning using Article Title: Massively parallel characterization and deep learning of enhancers in plant genomes Article Snippet: The plasmids were deposited at Addgene (Addgene no. 256230 and 256231; https://www.addgene.org/256230/ ; https://www.addgene.org/256231/ ). .. The CaMV 35S minimal promoter (−46 to +5 relative to the 35S transcription start site) followed by the 5′ UTR from a maize histone H3 gene (Zm00001d041672) and an 18-bp random barcode (VNNVNNVNNVNNVNNVNN; V = A, C, or G) downstream of an ATG start codon was cloned in front of the second codon of GFP by Golden Gate cloning using Clone Assay:Article Title: Massively parallel characterization and deep learning of enhancers in plant genomes Article Snippet: The plasmids were deposited at Addgene (Addgene no. 256230 and 256231; https://www.addgene.org/256230/ ; https://www.addgene.org/256231/ ). .. The CaMV 35S minimal promoter (−46 to +5 relative to the 35S transcription start site) followed by the 5′ UTR from a maize histone H3 gene (Zm00001d041672) and an 18-bp random barcode (VNNVNNVNNVNNVNNVNN; V = A, C, or G) downstream of an ATG start codon was cloned in front of the second codon of GFP by Golden Gate cloning using Article Title: Cis-regulatory architecture downstream of FLOWERING LOCUS T underlies quantitative control of flowering in Arabidopsis thaliana Article Snippet: .. In brief, sgRNAs were designed with CRISPR-P 2.0 [ ], annealed oligonucleotides were assembled into shuttle vectors via simultaneous restriction/ligation with Article Title: Developmental gene expression patterns driving species-specific cortical features. Article Snippet: Addgene #29687 pCS2-Flag-JUNB was used for JUNB overexpression experiments. .. Addgene #175573 pX458-Ef1adCas9-KRAB-MECP2-H2B-GFP (CRISPRi) was modified by replacing the Ef1a promoter with CAG using NEB HiFi DNA assembly reaction (NEB: E2621S). pCAG-CRISPRi plasmid generated in this study was then digested with Article Title: Cis-regulatory architecture downstream of FLOWERING LOCUS T underlies quantitative control of flowering in Arabidopsis thaliana. Article Snippet: .. In brief, sgRNAs were designed with CRISPR-P 2.0 [49], annealed oligonucleotides were assembled into shuttle vectors via simultaneous restriction/ligation with CRISPR:Article Title: Cis-regulatory architecture downstream of FLOWERING LOCUS T underlies quantitative control of flowering in Arabidopsis thaliana Article Snippet: .. In brief, sgRNAs were designed with CRISPR-P 2.0 [ ], annealed oligonucleotides were assembled into shuttle vectors via simultaneous restriction/ligation with Article Title: Cis-regulatory architecture downstream of FLOWERING LOCUS T underlies quantitative control of flowering in Arabidopsis thaliana. Article Snippet: .. In brief, sgRNAs were designed with CRISPR-P 2.0 [49], annealed oligonucleotides were assembled into shuttle vectors via simultaneous restriction/ligation with Modification:Article Title: Developmental gene expression patterns driving species-specific cortical features. Article Snippet: Addgene #29687 pCS2-Flag-JUNB was used for JUNB overexpression experiments. .. Addgene #175573 pX458-Ef1adCas9-KRAB-MECP2-H2B-GFP (CRISPRi) was modified by replacing the Ef1a promoter with CAG using NEB HiFi DNA assembly reaction (NEB: E2621S). pCAG-CRISPRi plasmid generated in this study was then digested with Control:Article Title: Developmental gene expression patterns driving species-specific cortical features. Article Snippet: Addgene #29687 pCS2-Flag-JUNB was used for JUNB overexpression experiments. .. Addgene #175573 pX458-Ef1adCas9-KRAB-MECP2-H2B-GFP (CRISPRi) was modified by replacing the Ef1a promoter with CAG using NEB HiFi DNA assembly reaction (NEB: E2621S). pCAG-CRISPRi plasmid generated in this study was then digested with |